Fig. 5

mTORC1 inhibition in microglia drives changes in their density and morphology after MCAO. A Representative immunohistochemical images from the entire dagame area (coronal section, 10 µm). Staining was performed used Iba-1 antibody. Scale bar: 25 µm. B Graphical representation of Iba-1⁺ cell counting and C graphical representation of Iba-1⁺ stained damage area. D Representative confocal immunofluorescence images from peri-infarct and infarct area. Staining was performed using Dapi (blue) and Iba-1 (red) antibody. Scale bar: 5 µm. E Graphical representation of the quantification of branching points and the F area occupied by each Iba-1⁺ cell in both damaged regions (white bars represent peri-infarct region whereas red bars represent infarcted area). Ordinary one/two-way ANOVA * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001. Unparied t-test ⁺ p ≤ 0.05; ⁺⁺ p ≤ 0.01; ⁺⁺⁺ p ≤ 0.001). Sham + V n = 5, Sham + R n = 5, MCAO + V n = 7; MCAO + Rpre n = 6 and MCAO + R_post n = 5 (V = vehicle; R = rapamycin). ⁺ vs Sham + V (peri-infarct region), ^# vs Sham + V (infarct region) and ^* vs same experimental group